BIACORE T200 PDF

Validation Using a high performance machine Good quality data can only be obtained with instruments that are in perfect working order. Regular maintenance of the system by the user and the service engineer will ensure that the machine is functioning properly and providing the highest quality data possible 1. The maintenance tools can be divided into three categories: : mechanical maintenance cleaning tubing and IFC leaving the instrument The following part is based on the Biacore , but will apply to most SPR instruments. Mechanical parts Starting with the mechanical maintenance, the first inspection involves the syringes, which provide the flow and deliver the sample. Syringes should not contain air bubbles and they must not leak. Then, an inspection of the injection port is done.

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Validation Using a high performance machine Good quality data can only be obtained with instruments that are in perfect working order. Regular maintenance of the system by the user and the service engineer will ensure that the machine is functioning properly and providing the highest quality data possible 1.

The maintenance tools can be divided into three categories: : mechanical maintenance cleaning tubing and IFC leaving the instrument The following part is based on the Biacore , but will apply to most SPR instruments. Mechanical parts Starting with the mechanical maintenance, the first inspection involves the syringes, which provide the flow and deliver the sample.

Syringes should not contain air bubbles and they must not leak. Then, an inspection of the injection port is done. Build-up of salt may occur and this must be washed away with water. The next step is to clean the vial dislodger the arm that holds the vials down. Clean it with a tissue with water. The final step involves the cleaning and positioning the injection needle. Wipe the needle with a tissue with water. For the best injections, the needle should go smoothly in the injection port.

Although it is not really a mechanical part, the photo-detector array that is used to determine the surface plasmon resonance angle should be checked for its performance 2. The actual curve is a fitting that enables the system to measure the resonance angle with great precision. Two abnormal dips are also depicted in the figure. Because the SPR angle is measured not at one point but in a larger section, surface heterogeneity will average the signal.

This results in the shallow dip-like curve 3. A large change in refractive index e. A slight, shallow dip should not be a problem. Flowing buffer at a high flow rate will push out tiny air bubbles. In addition, high protein immobilization will give a slight shallow dip pattern. However, for the best performance the sensor chip surface should be homogeneous and give normal dips. The other cleaning steps use chemicals that destroy proteins and can damage the sensor chip surface. Be sure to insert a dummy-chip before performing the following cleaning steps.

Desorb removes adsorbed proteins from the IFC and auto sampler. Some measurements will deviate in the first few runs after the DESORB command due to new adsorption of proteins to the tubing. It is possible to pre-coat the tubing with BSA assuming this will not interfere with the experiments that will follow.

Sanitize cleans the pumps, tubing and IFC, removing any micro-organisms. Superdesorb is a more thorough cleaning and is done as follows: Table: Superdesorb by Biacore.

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Product Support Biacore T is a versatile system for high-quality characterization of molecular interactions, from ions to viruses. One platform for comprehensive characterization of molecular interactions in terms of kinetics, affinity, specificity, comparability, concentration, immunogenicity, and thermodynamics Reliable ligand-binding assays, even for the most complex biologics Confident selection and characterization of the smallest organic compounds to large multidomain proteins Validated software meeting your regulatory expectations by supporting GxP procedures and 21 CFR Part 11 compliance Fast, simple kinetic analysis Biacore T software offers a range of tools for confident and reliable kinetic analyses. These analyses can be performed using a multicycle approach many samples against one ligand or when different ligands are immobilized , or alternatively, using singlecycle kinetics fast runs without regeneration. Read more Confident concentration analysis — with or without standards Software-supported direct binding and inhibition assays enable measurement of active concentration. The precision and automation of the system generates highly reproducible data. Calibration-free concentration analysis CFCA is of great value when no satisfactory calibration standard is available. Secure assays to meet regulatory needs The widely adopted Biacore T offers reliable ligand-binding assays—as described in regulatory guidelines—to characterize and assess comparability, even for the most complex biologics.

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